Journal: Molecular Therapy Oncology

Article Title: Integrating multi-omics analysis and machine learning to refine molecular subtypes and prognostic assessment of lower-grade glioma

doi: 10.1016/j.omton.2026.201227

Figure Lengend Snippet: Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in HS683 and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: The human LGG cell lines HS683 and H4, as well as the human embryonic kidney cell line HEK293T, were purchased from the American Type Culture Collection (ATCC).

Techniques: Expressing, Functional Assay, Biomarker Discovery, Western Blot, Knockdown, Migration, Wound Healing Assay, Cell Counting, Flow Cytometry

Journal: Journal of Korean Neurosurgical Society

Article Title: Spermine Synthase : A Potential Prognostic Marker for Lower-Grade Gliomas

doi: 10.3340/jkns.2024.0080

Figure Lengend Snippet: Knockdown of spermine synthase (SMS) inhibits the malignant behavior of lower-grade glioma cells. A : Western blot analysis of SMS protein expression levels in human normal glial cell lines CP-H121/122/123 and glioma cell line HS683/U251, with statistical analysis on the right. B : The efficiency of SMS knockdown in HS683/U251 glioma cell line detected by Western blot, with protein expression analysis on the right. C : The efficiency of SMS knockdown in HS683/U251 glioma cell line detected by quantitative reverse transcription polymerase chain reaction. D : Cell proliferation before and after SMS knockdown was measured by CCK-8 assay. E : The apoptosis ratio of cells before and after SMS knockdown was measured by flow cytometry, with statistical analysis on the right. F : Several cell clones formed before and after SMS knockdown, with statistical results. G : Migration of cells before and after SMS knockdown measured by scratch wound healing assay. H : Migration and invasive ability of cells before and after SMS knockdown measured by transwell assay. NC : normal control. *** p <0.001.

Article Snippet: The HS683 (HTB-138) human oligodendroglioma cell line and the U251 (HTB-17) glioblastoma cell line were obtained from ATCC (Manassas, VA, USA).

Techniques: Knockdown, Western Blot, Expressing, Reverse Transcription, Polymerase Chain Reaction, CCK-8 Assay, Flow Cytometry, Clone Assay, Migration, Wound Healing Assay, Transwell Assay, Control

Journal: Annals of Medicine and Surgery

Article Title: Unraveling the role of PBK in glioblastoma: from molecular mechanisms to therapeutic targets

doi: 10.1097/MS9.0000000000002708

Figure Lengend Snippet: Association between EIF4E and PBK and patient survival. The co-expression relationship between EIF4E and PBK was performed by the cBioPortal online platform (A). The expression of EIF4E was downregulated after PBK knockdown in HS683 (B). GEPIA online platform was used to perform the expression of EIF4E in glioma and its effect on survival. EIF4E mRNA expression in GBM compared to normal brain tissues (C) in which normal samples are marked in gray and GBM samples are marked in red. High EIF4E expression decreases both overall survival (D) and disease-free survival (E).

Article Snippet: The human glioma cell line HS683 was obtained from the American Type Culture Collection (ATCC) and cultured according to ATCC instructions in DMEM medium with 10% FBS in a 5% CO 2 incubator at 37°C. shRNA sequences for PBK knockdown and cell line establishment were based on a previous study .

Techniques: Expressing, Knockdown